Jacobs (groin) won't play in Friday's preseason game at Denver, according to Mike Spofford of the Packers' official site.
Jacobs returned to practice Tuesday after missing time with a groin issue, so Green Bay appears to be taking a cautious approach with its lead running back at this point during exhibition season. His absence Friday is notable for workload management, but he remains on track for regular-season duties if he avoids setbacks.