Mayer started at shortstop and went 0-for-4 in Thursday's 6-3 win over the Yankees.
The 23-year-old had opened the previous two games on the bench while dealing with a foot injury, but he played seven innings Wednesday in place of Caleb Durbin (finger) to prove his fitness. Mayer is expected to resume his role as the primary shortstop while Trevor Story (abdomen) works his way back from surgery.